Showing the last 30 topics from:

TopicRepliesViewsActivity
Cutadapt trim-paired with separate 5'/3' adapters retaining primers — is switching to linked adapters the correct fix?121Tue Jul 21 2026
Minimum length threshold in cutadapt for marker of variable length9110Mon Jul 20 2026
High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)2108Thu Apr 09 2026
Problem with cache using qiime cutadapt890Mon Mar 30 2026
Q2-cutadapt add "--nextseq-trim" option3108Fri Feb 27 2026
Possible bug in qiime cutadapt demux paired (2024.10)392Wed Jan 28 2026
Huge difference of DADA2 output with and without removing primers4109Tue Jan 27 2026
qiime cutadapt does not have an untrimmed sequences output4134Mon Dec 01 2025
ITS denoising truncation166Wed Oct 15 2025
Duplicate barcode error, but barcodes listed are not the same.697Tue Aug 19 2025
cutadapt outside of QIIME2164Mon Aug 04 2025
Inconsistent results with discard untrimmed reads in cutadapt6134Thu Jul 10 2025
QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis13513Thu Apr 17 2025
Analysing V4/V5 16S rRNA illumina Miseq paired-end reads.15430Tue Mar 18 2025
Trimming Paired-End Sequences with cutadapt While Maintaining Pairing Integrity185Mon Feb 10 2025
Which position is best for trimming in a paired-end approach?10162Fri Jan 24 2025
Comparing amplicon studies that use different primers6197Wed Nov 20 2024
Trimming 5bp in QIIME2 2024.5 Amplicon349Thu Nov 14 2024
Error messages after cutadapt, but got trimmed files.4207Thu Nov 07 2024
Cut adapt results in empty files3133Fri Oct 18 2024
One vs two adaptor sequences using cutadapt4107Fri Sep 27 2024
QIIME2 Demultiplexing Sequence6219Tue Sep 03 2024
V3-V4 16s - variable size after merging6266Mon Aug 05 2024
problem with cutadapt trim-single3144Tue Jul 02 2024
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt0186Thu Jun 13 2024
Removal of Illumina Adapters1204Thu May 30 2024
Error during deblur process7315Sat May 11 2024
Cutadapt - trimming adaptor and primer sequences2235Sat May 11 2024
Missing plugins to load Artifact to run cut adapt1146Tue May 07 2024
Primer show up again after removal using cutadapt in qiime2 DADA29416Mon May 06 2024